Biology A level planning experiment of effect of lead ions on amylase

Essay by Explosion March 2004

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The effect of Lead ions on amylase activity

Aim

What is the effect of Lead ions on the enzyme Amylase. And does it have an inhibitory effect, which causes the substrate, in this case starch to be blocked from the reaction process in the enzyme catalyst. Also is the effect reversible or irreversible, which is put on the amylase.

Method

Apparatus and substances required

Test tube holder 2% starch solution

6 boiling tubes labelled 1 to 6 1% lead nitrate solution

6 test tubes labelled A to E, ( F) Dimple tiles

5 test tubes labelled A1, B2, C3, D4, E5, (F6) 5 ml syringe

1% Amylase solution 4 dropping pipettes

Stop clock

Firstly, in each of the boiling tubes place 8 ml of 2% starch solution using the syringe. Then in each of the test tubes labelled A to F add 1ml of 1% Amylase solution. In the remaining 6 test tubes add the different concentrations of the lead nitrate solution as shown below in their corresponding test tubes.

All concentrations are achieved by serial dilution originally coming from a 1% lead nitrate solution.

Overall

Concentration of Lead nitrate in solution

A1 - 1ml lead nitrate solution (1%) 1%

B2 - 0.8 ml of 1% lead nitrate solution, 0.2 ml of distilled water 0.8%

C3 - 0.6 ml of 1% lead nitrate solution, 0.4 ml of distilled water 0.6%

D4 - 0.4ml of 1% lead nitrate solution, 0.6 ml of distilled water 0.4%

E5 - 0.2 ml of 1% lead nitrate solution, 0.8 ml of distilled water 0.2%

F6 - N/A N/A

After these are all measured out accurately fill dimple tiles with iodine solution. To start the reaction mix the two test tubes, A and A1 in to boiling tube, 1mix well and start the clock, and every...