Discussion:In this experiment, the control tube is the one that transmit light the most compare to the other eleven tubes, and that increasing the concentration will affect the conductivity of the solution. So as the solution becomes more concentrated, the solution seems to have higher conductivity.
The osmolality of red blood cell is 330mOsm. Salt concentration is proportional directly with osmolality, and both osmolality and salt concentration show an inverse relation with hemolysis. So the lower the salt concentration, the higher the hemolysis will be, and the lower the osmolality the higher the hemolysis, and vise versa. So one would expect the percent of hemolysis to decrease as the salt concentration increases from 0 %( control tube concentration) to 1.1%While performing this experiement, we encoutered few unexpected data. So we expected that we made few mistakes while performing the experiment. However, many other factors can play a rule in causing an error if it wasnot accounted for, for example: whether the blood was fresh or old, and whether the blood PH was high or low.
So we expect to have higher oxygen affinity when blood comes from young sheep than old one. Also the PH level will play an important role in getting the correct results, because increasing blood PH will increase oxygen affinity; so we would get different results if the blood used in our experiement was acidicIn this experiment, we have seen the responses of red blood cells to varying concentration and osmolality. We have also seen the relationship between absorbance and hemolysis, and between osmolality and hemolysis. One experiment I will like to perform in the future is the effect of losing nucleus in red blood cells on oxygen concentration capacity. Losing the nuclues.
enables the red blood cell to contain more oxygen-carrying haemoglobin, thus enabling...