1.Problem / Purpose
The purpose of this experiment is to investigate the properties of an enzyme - substrate reaction
Hydrogen peroxide is a very reactive compound that can be used for a variety of reactions including bleaching and disinfecting minor wounds. Acting as an oxidizing agent, it is also toxic to cells, hence its value as a disinfecting agent that disrupts the metabolism of bacteria. Our body cells and those of many other animals contain an enzyme called catalase that accelerates the conversion of toxic hydrogen peroxide to water and oxygen gas.
In this experiment, if the concentration of the enzyme is increased, then the reaction will proceed faster and if the concentration of the enzyme will decrease, then the reaction will proceed slower.
All the materials used in the experiment are presented on the lab sheet
The instructions for the experiment are presented on the lab sheet
[cc]AMOUNT OF mL OF GAS EVOLVED AT THE END OF ...TOTAL EVOLVED
10 s20 s30 s40 s50 s60 s90 s120 s
SUBSTRATE: Hydrogen Peroxide (H2O2 (l))
PRODUCTS: Water (H2O(l)) and Oxygen gas (O2 (g))
From theory, at STP, 1 mol of a gas is equal to 22400 mL or 22400 g
( ) = m (1.2)
where: ÃÂÃÂ® The number of moles of Hydrogen Peroxide: = ;
ÃÂÃÂ® The molar mass of Hydrogen Peroxide: = ;
ÃÂÃÂ® The mass of Hydrogen Peroxide: m ;
2 mol = 64.08 g
( ) = m (1.3)
where: ÃÂÃÂ® The number of moles of Oxygen gas: = ;
ÃÂÃÂ® The mass of a gas at STP:...