The Effect of Enzyme Catalysis Concentration On the Substrate Reaction Rates Name here Biology October 21, 2001 Enzyme Concentration 2 Abstract Hydrogen Peroxide 1.5% was germinated with different levels of enzyme catalase concentrations from 100% enzyme to 0% enzyme, adding water to dilute the concentrations. The reaction rate with 100% enzyme concentration, opposed to the other levels of enzyme concentration, increased the catalystic reaction rate. These results maybe significant if the use of the timing mechanism is not precise. This would alter your final data of how long the enzyme reaction rate took place.
Introduction Enzymes are proteins produced by living cells and act as catalysts (which affects the rate of a chemical reaction) in biochemical reactions. One consequence of enzyme activity is that cells can carry out complex chemical activities at relatively low temperatures. (AP Lab 2 Enzyme Catalysis & AP Biology Book 2001) In an enzyme-catalyzed reaction, the substrate binds reversibly to the active site of the enzyme.
This in turn allows a reduction in the energy required to activate the reaction of the substrate molecule so that the products of the reaction are formed.
In the absence of catalase, the reaction occurs spontaneously, but very slowly. Catalase speeds up the reaction considerably. By studying the kinetics of the enzyme-catalyzed reactions you can learn a lot about enzymes as a whole. For example, (AP LAB 2 2001) it is possible to measure the amount of product formed, or the amount of substrate used, from the moment the reactants are brought together until the reaction has stopped.
Enzyme Concentration 3 Materials and Methods Enzyme Catalase was prepared using chicken liver, and put on ice so the enzyme would not denature. I poured 10-mL of 1.5% Hydrogen Peroxide into a clean 50-mL glass beaker. A punch hole sized filter paper...