Pro-apoptotic Effects of Carvacrol on Human Metastatic Prostate Cancer Cell Line, LNCaP

Essay by narender0026University, Master'sA, September 2014

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Pro-apoptotic Effects of Carvacrol on Human Metastatic Prostate Cancer Cell Line, LNCaP

A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE

WITH SPECIALIZATION IN PHARMACOLOGY/TOXICOLOGY

TO THE FACULTY OF THE ARNOLD & MARIE SCHWARTZ COLLEGE OF

PHARMACY AND HEALTH SCIENCES,

LONG ISLAND UNIVERSITY, BROOKLYN, NY

8th Aug 2014

BY

THEJA KANDRA

SPONSORING COMMITTEE

_______________________

Supriya Bavadekar, Ph.D., Committee chair

_______________________

Warren Ratna, Ph.D., Committee member

_______________________

Sandeep Vansal, Ph.D., Fairleigh Dickinson University's

School of Pharmacy, Committee member

_______________________

Rutesh H. Dave, Ph.D., Division Director

_______________________

Martin E. Brown, M.S., Associate Dean

ABSTRACT

Carvacrol, a monoterpene phenol, present as an active constituent of the volatile oil of oregano and thyme, has been shown to have anti-proliferative effects on various human cancer cell lines, such as breast cancer, hepatoma, and cervical cancer. However, its effect on prostate cancer cells has not yet been determined.

The objective of this research was to investigate the pro-apoptotic effects of carvacrol on an androgen-sensitive, human, metastatic prostate cancer cell line, LNCaP. In this study, the effect of carvacrol on the pro-apoptotic factors like caspase-3 was determined using the Caspase-3 Colorimetric assay. The results obtained showed a clear-cut involvement of the caspase-3 activity in the cell death. Also, the role of Bcl2 Family in regulating the apoptosis has been determined by the western blot analysis of pro-apoptotic and anti-apoptotic proteins i.e., Bax/Bcl2 ratio, which showed a significant increase in the ratio suggesting the cell death to be apoptotic. Furthermore, the involvement of NfKB translocation has been studied to understand the probable mechanism of cell death caused by carvacrol. Investigations suggested that carvacrol's anti-proliferative action is independent of NF-κB translocation. This is the first study that has identified the pro-apoptotic effects of carvacrol in human...