Steam Distillation of Eugenol from Cloves.

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Introduction: This experiment involves an extraction of a natural product using the techniques of steam distillation. The principle component of oil of cloves is an aromatic compound, which is identified by thin layer chromatography (TLC). Eugenol is widely used in dentistry, due to its analgesic, antiseptic balsamic qualities. It is ideal for curative for pulp hyperemia (the soft, sensitive substance underneath the enamel and dentine of a tooth) and pulp its. In dentistry eugenol is also used to cover cavities, fill canals, etc. It also keeps food fresh and kills off bacteria and viruses. Eugenol helps keep meat fresh and prevents stomach upset, diarrhea, and wound infections. In addition, it might play a part ...

... up and distil off most of the dichloromethane solvent. 9. The remaining solution of Eugenol in Dichloromethane can be removed using a disposable pipette and placed into a clean pre-weighted vial. 10. The vial can be heated on a steam bath under the hood to remove the small amount of dichloromethane. 11. The pure Eugenol should be weighted to determine the percent oil recovered based on the mass of cloves originally used. Results: Density of Eugenol: 1.34 g/mL BP of Eugenol: 40°C Initial mass of Eugenol: 18.545 g Mass of vial: 18.0063 g Mass of Eugenol Oil: 18.434 g % Recovery = (18.545 g - 18.434 g)/18.434 x 100 = 0.602% Thin layer chromatography: Rf=(Distance substance moved/Distance solvent moved) Rf=(4.2/5.1) Rf=(.824) Conclusion: The experiment was successful in that we did isolate eugenol

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