Essay by PaperNerd ContributorCollege, Undergraduate February 2002

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Introduction: This is an experiment testing spontaneous generation. The hypothesis is that living things will not spontaneously generate from non-living things. The variable is that one of the samples is exposed to the air while the other sample is not.

Materials and methods: The materials needed are two flasks, a cotton stopper, and enough nutrient broth to put in each flask. Take both flasks and fill both of them with the nutrient broth. Put a cotton stopper in each of them. Then autoclave both of the flasks. Take the stopper out of one of the flasks. Let the flasks sit for one week and then make observations.

Results: The result was that the control, the flask containing the cotton stopper, appeared to be the exact same as it had the week before. The experimental flask, the one without the stopper, had mold-like spores growing in it and it had changed to a cloudy yellow color instead of the clear yellow that it was the week before.

Discussion/Conclusion: The hypothesis was confirmed. It was proved correct because the flask containing the cotton stopper did not contain any living things. An experiment that would present similar results would be if you took two fully cooked pieces of meat, that way there are still nutrients but nothing is living on it, and put one of them in an air tight seal. Let the other one remain in the open air. After letting them sit for a while, the same observations could be made.