03 - 31 - 2006
Results:
In this experiment the effect of different hormones on heart rate of Daphnia was examined. It was investigated which substances act as stimulants and which substances act as depressant. Initially, Daphnia was placed on depression slide under the microscope and the average heart rate was determined. Subsequently, Daphnia was treated with different type of hormone and the effect on Daphnia heart rate was observed. It was investigated which substances act as stimulants and which substances act as depressant. Daphnia was treated with aged water, caffeine, ethyl alcohol, nicotine, and epinephrine, and their effect on heart rate of Daphnia was observed. The results are posted on table 1:
Table 1: Average heart rate for Daphnia at pre-stimulus and post stimulus for different hormones:
# of Try | Aged Water | Caffeine | Ethyl Alcohol | Nicotine | Epinephrine | |||||
Control | Treated | Control | Treated | Control | Treated | Control | Treated | Control | Treated | |
1 | 277.2 | 278 | 285.2 | 343.2 | 297.2 | 266.8 | 318.5 | 274 | 298 | 344.8 |
2 | 204.68 | 200.67 | 189.3 | 272 | 228 | 171.3 | 270.67 | 238.67 | 298.67 | 324.67 |
3 | 273.3 | 289.3 | 232.7 | 262.7 | 234 | 222 | 244 | 126 | 252 | 295.3 |
4 | 264.67 | 194 | 269.33 | 324.67 | 258 | 167.33 | 262.67 | 272.67 | 258.67 | 184 |
5 | 360.3 | 283.4 | 283.7 | 310.4 | 279.1 | 235 | 274.1 | 339.6 | 283.6 | 252.2 |
6 | 204 | 260 | 272 | 328 | 328 | 228 | 244 | 160 | 276 | 340 |
7 | 285 | 324.7 | 285 | 335.3 | 283.3 | 299.3 | 282.7 | 270 | 276.7 | 299.3 |
8 | 264 | 259 | 279 | 300 | 295 | 296 | 299 | 294 | N/A | N/A |
Average | 266.643 | 261.134 | 262.029 | 309.538 | 275.325 | 235.716 | 274.455 | 246.868 | 277.663 | 291.467 |
Standard Deviation | 49.337 | 44.373 | 34.068 | 29.426 | 33.734 | 50.310 | 25.687 | 70.690 | 17.857 | 56.964 |
P value | P>0.05 | P<0.05 | P>0.05 | P>0.05 | P>0.05 |
During initial observation, Daphnia was placed in microscopic slide and the average heart rate was calculated.
Then, aged water was added to Daphnia and its effect on heart rate of Daphnia was observed. The...