Enzymes

Enzymes

Experiment #1- Effect of concentration of enzyme on rate of reaction.

Variables- Concentration.

Purpose- To find the amount of gas produced by different concentration of peroxides

decomposing.

Hypothesis- The higher the concentration of the peroxidase the more gas will be produced.

Experiment #2- Effect of temperature on the rate of reaction.

Variables- Temperature.

Purpose- To determine if the peroxidase will be effected by temperature.

Hypothesis- Peroxidase treated with higher temperature will produce more gas until 38 degrees

C, then less gas will be produced.

Experiment #3- Effect of pH on the rate of reaction.

Variables- pH levels.

Purpose- To find the effect of the pH ranges on the peroxidases' ability to decompose peroxidase.

Hypothesis- The lower the pH the more gas produced.

Procedure- All the labs used set a special experimental design. This design consisted of a pan

containing water, reaction vessel and an inverted graduated cylinder. The diagram below (figure-1) further explains the parts of the design.

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(note all three experiments use the same set up design but had different variables specific to each experiment.)

figure-1

Concentration experiment- For this and every experiment the this procedure was repeated except for some variables specific to each individual experiment. First a cow liver was chopped up to serve as the enzyme of peroxidase. (That procedure was only performed once and used for all three labs to keep the same concentration) Then whatman's #1 filter paper was hole-punched to serve as the disks. Then theses disks were soaked in the enzyme mix, and these were the enzyme soaked disks. Next four disks where placed inside the reaction vessel, and 10 ml. of peroxide was also poured into the vessel. Immediately following, the stopper was used to cap the vessel, however making sure that the peroxide did not come in contact...