Flora Lebiti
10/18/14
Micro 235
Oxidase Test Lab Report 5-6
Observation:
In the Oxidase Test it is designed to identify the presence of cytochrome c oxidase. Designed to differentiate bacteria based on their ability to respire. It is able to do this because cytochrome c oxidase has the unique ability to not only oxidase cytochrome c, but to catalyze the reduction of cytochrome c by a chromogenic reducing agent called tetramethyl-p-phenylenediamine. In the test the reducing reagent is adding directly to bacterial growth on solid media, or a bacterial colony is transferred to paper saturated with the reagent. My experiment will include the organisms E.Coli, M. Catarrhalis and oxidase.
Hypothesis:
This test is to identify bacteria containing the respiratory enzyme cytochrome c oxidase. It can be used to determine the difference of negative and positive. My hypothesis is that all of them would turn a different color because the reagent on the paper is added to a system in order to bring about a chemical reaction (to see if anything changes).
By turning blue I believe they will all be positive as well. This does not mean they will change, it is just my educated guess.
Experiment:
Read directions about experiment on the exercise sheet, page 168
Listen and watch professor perform the experiment, and ask questions before starting it on your own
Put on safety gear (lab coat, gloves, put hair up)
Grab materials. These include a plastic loop (your need three loops because using three different organisms), reagent slide, fresh slant or plate of E.coli, M. Catarrhalis and Oxidase.
Label each square on the reagent slide with the organism so you don't get confused on which box is which organism
Following your instructor's guidelines, transfer some of the culture to the reagent slide (one organism at...