Essays Tagged: "E. Coli"

Plasmid Fusion and PCR

on and PCR. Each one is a complicated procedure of geneticengineering, with our own cheek cells and E.Coli supplied by AMGEN. I will start byexplaining the Plasmid Fusion lab.The Plasmid Fusion lab co ... working with are plasmids. Plasmids are gene sequences found in aloop outside of the main chromosome. Their main purpose is to code enzymes that digestantibiotic enzymes. Antibiotics are chemicals th ...

(5 pages) 64 0 4.2 Dec/1996

Subjects: Science Essays > Biology

Growth Dynamics of E. coli in Varying Concentrations of Nutrient

AbstractThe purpose in this experiment of growth dynamics of E. coli in varying media was to determine which media produces the maximum number of cells per unit ... which media produces the maximum number of cells per unit time. First a control was established for E. coli in a 1.0x nutrient broth. This was used to compare the growth in the experimental media of 0 ... readings were taken and calculations made to determine the number of cells present after a given time. Then two graphs were plotted, Number of cells per unit volume versus Time in minutes and Log of t ...

(10 pages) 88 0 4.6 Apr/1997

Subjects: Science Essays > Biology

Escherichia coli

erium is so common that its name has come to describe all similar bacteria as "coliform" or coli-like.These enterobacteria are gram-negative, nonspore-forming straight rods, aerobic and facultative an ... mm in length. All species ferment glucose, which allows for a testing method known as "presumptive E. coli," and all microscopically, are indistinguishable. Most species are motile with nonpolar flag ...

(4 pages) 96 0 2.7 Apr/1996

Subjects: Science Essays > Biology

Myth about why people are fat

ut Gluttoneus never had left people starving.Foodisus instantaneously visited Starvisus's village. His village was pretty small, no more than 100 people. It was not even close to the number of peo ... ost beloved foods. Foodisus immediately took Gluttoneus's whole supply of food to Starvisus's village. The village chomped down on it like there was no tomorrow. They were overjoyed. But in order that ...

(2 pages) 54 0 4.7 Dec/2002

Subjects: Social Science Essays > Society and community

This is an article analysis of Jack in the Box

pany Inc., including, making recommendations for improvement for my organization based on the article. Lastly, explain the importance of ethics in accounting and financial decision making.Although Jac ... n the Box remained strong with widely varied menu items and unique marketing strategies through the E. coli disaster of 1993, the current economic slump in the fast food industry highlights serious co ...

(3 pages) 264 0 3.6 Feb/2004

Subjects: Businesss Research Papers > Accounting

Is Our Food Safe? Includes Bibligraphy

oods, to determine their relationships to health, digestions and to create guidelines as to there use. Given the many other potentially harmful preservatives, the "poison squad" experiments, tested th ... ilk). The number of cows effected with clinical mastitis and the number of cases per cow may increase. In addition, the risk of sub-clinical mastitis (milk not visibly abnormal) is increased. In some ...

(6 pages) 96 0 0.0 Mar/2005

Subjects: Social Science Essays > Current Issues


leus of each human cell, there are 46 chromosomes, and each chromosome contains a single DNA molecule. For comparison, in the bacterial species Escherichia coli, each cell has only one chromosome and ... bacterial species Escherichia coli, each cell has only one chromosome and thus only one DNA molecule.Regardless of the organism, every molecule of DNA is constructed of two parallel, ribbons like cha ...

(1 pages) 45 1 3.0 Apr/2005

Subjects: Science Essays > Biology

Information on the biopolymer poly-hydroxybutanoate (PHB)

b by feeding bacteria a diet rich in nutrients until large colonies form and then withdrawing glucose. The bacteria immediately start secreting PHB, which provides them with an energy store. In the 19 ... s needed for the production of PHB were successfully cloned and transferred into a common bacteria, E.coli because it reproduced quickly and has an easily manipulated physiology. The PHB gene is trans ...

(2 pages) 30 0 4.8 May/2006

Subjects: Science Essays > Chemistry

AP Bio pGLO Transformation Formal Lab Report

Abstract:The topic of this research involved the occurrence of genetic transformation in bacteria (E. Coli). More specifically, a previously prepared pGLO plasmid--which consisted of the gene to be c ... oduce no bacteria colonies, as it wouldn't be able to grow without the gene for ampicillin resistance. Also, the transformed solution with just LB and ampicillin would produce bacteria colonies but th ...

(5 pages) 205 1 3.3 May/2006

Subjects: Science Essays > Biotechnology

Is Food Irradiation safe?

on is the emergence of new types of harmful bacteria or evolving forms of old ones that cause disease. Food Irradiation is a great new process that can eliminate disease- causing microorganisms such a ... . When food is irradiated the nutritional value is unchanged and the food does not become radioactive. Similar technology is used to sterilize medical devices so they can be used in surgery or implant ...

(6 pages) 56 1 4.0 Jun/2006

Subjects: Science Essays > Biotechnology

Analysis of Purified rGFP Using SDS-PAGE gel/Coomassie - Blue and Western Blot

eristics. The GFP gene can be cloned and reconfigured to be expressed in numerous organisms such as E. coli to induce its expression for purification and analysis. The rGFP produced can then be purifi ... using Bradford assay. Purity analysis of the rGFP obtained was done using SDS-PAGE gel/Coomassie Blue. Finally a Western Blot was performed to confirm the protein present is actually the protein of in ...

(11 pages) 53 0 1.0 Apr/2007

Subjects: Science Essays > Biotechnology

E. Coli

E.Coli E. Coli is a bacterial strain that affects many people around the world each year. I ... und out that it also causes urinary tract infections. The article has stated this "strain" of E. Coli is drug resistant which means that a typical course of antibiotics will not clear up the inf ... ere the same among the women. Urinary tract infections are common among women and 90% are caused by E.Coli.When the strain is traced to one or more sources, many steps will be taken to prevent it from ...

(1 pages) 665 0 0.0 Jan/2002

Subjects: Businesss Research Papers

Walkerton Crisis

n on the map, and maybe a great place to visit, that though has changed forever. A deadly strain of E. coli originating from cow manure contaminated the town's water supply. The E. coli contamination, ... . When washing hands, even with soap, bleach must be used to ensure that the deadly bacterium is gone. It isn't even safe to have a bowl of soup unless it is made from bottled water. No one could have ...

(3 pages) 6 0 0.0 Nov/2001

Subjects: Literature Research Papers

Bacteria In General: Brucellosis

" they may be small but can be found in every environment or habitat in our known world and existence. They are the means by which some life exists, some people get sick, the way certain things smell ... to become accustomed to their new environment. Usually there is little or no growth during this phase. Next comes the Log phase, their growth begins and the rate of multiplication is the most rapid an ...

(9 pages) 46 0 4.6 Sep/2007

Subjects: Science Essays > Biology


athogen is lets name a few. 1. Bacteria in the form of, Arcobacter Butzleri Helicobacter Pylori And E. Coli 2. Viruses Rotaviruses and Adenoviruses Type 40 and 41 3. Protozoa Acanthamoeba. Each of the ... ing from and stop it. Furthermore educate your workers about the problem. Education never hurt anyone. In conclusion the food industry is greatly wronged by the general public when it comes to identif ...

(3 pages) 11 0 0.0 Feb/2008

Subjects: Science Essays > Biology

Iago's Motives In Othello

easons that the Escherichia coli did not produce the interferon after being transformed. First, the E. coli cells might not have accepted the plasmid during the transformation process. If the electrop ... nd not have induced micropores in the cell membrane large enough for the uptake of foreign DNA. For E. coli the voltage range for successful transformation by electroporation is 250 to 4,000 V/cm. Vol ...

(8 pages) 17 0 0.0 Feb/2008

Subjects: Science Essays > Biology

Foodborne Competition.

own groceries; while some choose to go out to eat or to purchase something ready to eat to bring home. No matter what choice is made there is always the risk for possible infection or foodborne illnes ... illnesses have been around ever since people decided to eat food, which is probably a very long time. Foodborne illness often presents itself as flu-like symptoms such as nausea, vomiting, diarrhea, ...

(9 pages) 60 2 4.6 Feb/2008

Subjects: Humanities Essays > Health & Medicine

Microbiology lab report on Escherichia coli and Staphylococcus aureus.

different agar types were used in this experiment. The first (Nutrient) allowed for growth of both E. coli and S. aureus. The second agar used (MKL) inhibited the growth of S. aureus but allowed the ... d S. aureus. The second agar used (MKL) inhibited the growth of S. aureus but allowed the growth of E. coli. The third (ASH) yielded growth of both bacterial species. The fourth (Mannitol) was found t ...

(3 pages) 87 0 5.0 Sep/2008

Subjects: Science Essays > Microbiology

DNA Experiments

e 3 classical experiments and indicate how each provided evidence for the chemical nature of the gene.a. Hershey and Chase- bacteriophage replicationThey prepared two separate samples of viruses, one ... ch the protein (contains some sulfur and no phosphorus) was labeled with a radioactive isotope 35S. E. coli were grown on a medium that contained one of the radioactive isotopes. One colony of bacteri ...

(2 pages) 16 0 0.0 Feb/2009

Subjects: Science Essays > Biochemistry

Treatment of drinking water and the processes used in the Illawarra region. HSC year 12 syllabus

temperatures can kill bacteria that can be present in water. It can be used to kill an outbreak of E. coli to help prevent sickness from tap water.To check that there are no hot or cold discharges in ... tion with a complexing agent (EDTA) and indicator is used to determine the hardness of a water sample. Stoichiometry: Ca2+/EDTA = 1:1Reaction = caTurbidity Is a measure of the amount of colloidal susp ...

(19 pages) 25 0 0.0 Apr/2009

Subjects: Science Essays > Chemistry