Essays Tagged: "Polymerase chain reaction"

PCR And Its Use

ology lab to detect pathogens. It relies upon amplification technology utilizingthe heat stable DNA polymerase from a thermophilic organism.' (from Dr. K.Mullis ...

(3 pages) 84 0 3.0 Jan/1997

Subjects: Science Essays > Biology


peats'. These extra chemical units give everyone's DNA a unique pattern. The newer method is called Polymerase Chain Reaction (PCR). This system uses an enzyme that can be directed towards regions of ...

(5 pages) 174 0 4.6 Nov/1997

Subjects: Science Essays > Genetics & Genome Projects


IntroductionPolymerase chain reaction (PCR), can be used to amplify rare specific DNA sequences into many billio ... nthesis, which begins upon addition of a supply of deoxynucleotides and a temperature-resistant DNA polymerase such as that from Thermus aquaticus (a bacterium that lives in hot springs). The Taq poly ... etween the primer sites is doubled; therefore, the desired sequence increases exponentially (2, 16).Polymerase Chain Reaction (PCR)Polymerase Chain Reaction (PCR)The elegant technique of PCR, by which ...

(22 pages) 278 2 3.0 Dec/2002

Subjects: Science Essays > Biotechnology

DNA Microarray Technology. Basics of the DNA chips. Applications of DNA chips.

obots.5.RNA Isolation: For prokaryotic cells, first of all the cell extract is treated with poly(A) polymerase, and polyadenylation of mRNA is maintained. All eukaryotic mRNAs are naturally polyadenyl ... is maintained. All eukaryotic mRNAs are naturally polyadenylated, thus there is no need for poly(A) polymerase treatment. By oligo d(T) chromatography, the mRNA molecules are isolated.6.Total RNA labe ...

(6 pages) 98 0 5.0 Mar/2003

Subjects: Science Essays > Genetics & Genome Projects

DNA Fingerprinting Vocabulary List.

short, single-stranded DNA molecule bound to a fluorescent dye that makes the probe visible·Polymerase Chain Reaction (PCR): technique that allows molecular biologists to make many copies of a ...

(1 pages) 36 0 3.0 May/2003

Subjects: Science Essays > Biotechnology

Mutation in gene Unc-93 results in apparent muscle defects in C. Elegans.

hat N2 is the wild-type worm since its DNA had no deletions. Primers were used in a process called Polymerase Chain Reaction (PCR) to amplify regions on the unc-93 gene in order to determine the dele ... legans LU-55, LU-57, and N2 were purified to examine if a mutation was present in the unc-93 gene. Polymerase Chain Reaction (PCR) was used to amplify these purified samples. Finally, the sizes of t ...

(11 pages) 51 0 5.0 Dec/2003

Subjects: Science Essays > Biology

Polymerase Chain Reaction (PCR)

Dr. Kary Mullis first described polymerase chain reaction (PCR) in 1983, and won the Nobel Prize in chemistry in 1993 for his work p ... genetic code can occur. It is because of the research, laboratory work, and new techniques, such as polymerase chain reaction, that have come along that have helped bring about the knowledge of the hu ... ich uses DNA manipulation to produce many copies of the gene or segment of DNA. A third way is with polymerase chain reaction or PCR. This technique or concept allows scientists to amplify a specific ...

(12 pages) 133 0 4.2 Feb/2004

Subjects: Science Essays > Chemistry

Fragile X Syndrome

n be determined for both affected individuals and suspected carriers. Two main approaches are used, polymerase chain reaction (PCR) and Southern blot analysis. PCR analysis utilizes flanking primers t ...

(5 pages) 61 0 4.3 May/2004

Subjects: Social Science Essays > Psychology

BioTechnology Essay

Vidushi SinghAP Biology pd. 2March 16, 2004Biotechnology Essaya) PCRPCR- Polymerase chain reaction is a method used to quickly and selectively amplify pieces of DNA instead ... ufficient or tainted. The initial DNA is incubated in a test tube with a heat-resistant type of DNA polymerase, a supply of all 4 nucleotides and primers. The primers are complementary to the ends of ... briefly heated to separate the DNA strands, then cooled so that the primers will hydrogen-bond. DNA polymerase now comes in handy; it adds nucleotides to the 3' end of the each primer. Each such cycle ...

(3 pages) 62 0 5.0 Jun/2004

Subjects: Science Essays > Biology

DNA profiling

sample, and takes less time than RFLP. The DNA sample can be "amplified" by using a process called Polymerase Chain Reaction (PCR), but this is lees precise than RFLP.DNA molecules are able to make c ... s precise than RFLP.DNA molecules are able to make copies of themselves. By adding an enzyme called polymerase to the DNA sample, then placing it in a device called a thermocycler, scientists create a ...

(3 pages) 80 0 4.0 Oct/2006

Subjects: Science Essays > Genetics & Genome Projects

Uses of DNA and Fingerprints in Crime Scene Investigation

illionth of a gram) for the test. This step takes about one to two hours.The third step is known as Polymerase Chain Reaction (PCR). Certain chemicals are added to make specific parts of DNA reproduce ... ing and cooling cycles needed to be run to be able for the DNA to be used to run against other DNA. Polymerase Chain Reaction takes approximately three to five hours.Step four is known as Typing. A ma ...

(5 pages) 66 0 3.0 Jan/2008

Subjects: Science Essays > Biology

The Pertussis Outbreak

eightened awareness of pertussis among healthcare providers, increased reporting and routine use of polymerase chain reaction (PCR) testing for the diagnosis (Broder, et al., 2006).Long considered a c ...

(4 pages) 28 0 5.0 Jan/2008

Subjects: Humanities Essays > Health & Medicine > Diseases