Cold Shock Response and Hyphal Growth Rate of Neurospora crass, exposed to Various Time Phase at 4oC.
ABSTRACT
The effects of cold-shock at 4oC and hyphal growth rate on Neurospora crassa were investigated in this study. The mechanisms responsible for controlling hyphal extension and branching at various conditions are still poorly understood. Our experiment was trying to find the relationship between the hyphal growth rate and cold-shock. In addition, the protein analysis at different time phase of cold-shock was also being compared. Length of a selected hyphal tip in 20 minutes of Neurospora crassa has been measured after 0 min cold-shock (control), 45 min cold-shock and 90 min cold-shock in 2 separate trials. In trial one, the mean growth rate of 0, 45, and 90 min cold-shock were 24.4, 14.6, and 9.53 um/min respectively. In trial two, the mean growth rate of 0, 45, and 90 min cold-shock were 33.7, 9.13, and 5.92 um/min respectively. The results showed that there was a significant difference between the hyphal growth rate and cold-shock on Neurospora crassa and it appeared that longer the cold-shock, slower the hyphal growth rate. It is concluded that various time phase of cold-shock reduced the metabolic activity level and therefore, inhibits the hyphal growth rate of Neurospora crassa.
INTRODUCTION
Many factors could affect the metabolic level of Neurospora crassa, but among other factors, cold-shock exerts an important constraint on hyphal growth rate of Neurospora crassa (Park et al. 1997). Also, the mechanisms responsible for controlling hyphal extension and branching at various conditions are still poorly understood (Watters et al. 2000). Therefore, the trend of Neurospora crassa branching under different time phase of cold-shock will also be observed in this experiment. In addition, the branching of Neurospora crassa leads to increased secretion of certain proteins (Lee et al. 1998). As a result, the protein extraction will be performed for one of the trials.
The study of different...
More Biology
essays:
An Investigation To Discover Whether Or Not There Is A Relationship Between Light Intensity And The Surface Area Of A Leaf.
... this shows that there is no significant relationship between the light intensity and the area of the leaf, from my results. My results could be inconsistent, as I may not have been able to control properly ...
Darwin's analogy with artificial selection to show that natural selection occurs in our environment.
... was that natural selection is the main, but not exclusive, mechanism for this divergence of lineages. Proof for Darwin's first argument of "decent with modification" came from his analysis in ...
A comparison of the respiration of yeast in different sugar substrates.
... strong and reliable results, the conclusion obtained will be strengthened, showing that there is a faster rate of CO2 production when yeast respires ...
The Effect Of Substrate on Enzyme concentration
... burette by upward delivery through the delivery tube. 10. Assume that time = 0 that there is 0 cm3 oxygen collected in the gas burette i ...
Biology Coursework Analysis and Evaluation (transpiration)
... the rate of transpiration. My results were accurate enough to show that there was strong correlation between the possible factor (light intensity) and ...
West Nile Virus: What is it and how did it get here?
... will show that there is evidence to prove that the government has done just that in years past. There are facts that prove that the ...
Bioethics
... they do not provide sufficient information. The history of medicine shows that there has always been a need for experimentation on human beings ... given protection by the Food and Drug Act. These laws control new medications into the market. Although this seems as though ...
Prader-Willi Syndrome
... some of the common features of PWS. Recently, studies have show that there is a strong link between this and sleep quality. Some ... to gain weight. To keep the baby's weight under control supervision by a professional nutritionist or a specialist who understands ...