TESTING FOR MACROMOLECULES
To determine the presence of glucose, starch, lipids, and protein in various samples.
ÃÂ·6 graduated cylinders
ÃÂ·6 test tubes
ÃÂ·A test tube rack
ÃÂ·hot water bath
ÃÂ·test tube brush
ÃÂ·40 mL protein solution
ÃÂ·40 mL vegetable oil
ÃÂ·40 mL maltose solution
ÃÂ·40 mL sucrose solution
ÃÂ·40 mL starch solution
ÃÂ·40 mL distilled water
ÃÂ·Benedict's solution in a dropper bottle
ÃÂ·Iodine solution in a dropper bottle
ÃÂ·Sudan IV solution in a dropper bottle
ÃÂ·Biuret reagent in a dropper bottle
ÃÂ·Glass ware soap
1. Hot water bath was set up by using medium setting for the hot plate.
2.The six graduated cylinders were marked with number 1 to6.
3.Six-test tubes were also marked with the number 1 to 6.
4. Protein solution was measured out 10 mL and was poured into graduated cylinder 1.
10 mL of vegetable oil was poured into graduated cylinder 2, 10 mL of maltose solution into graduated cylinder 3, 10 mL of sucrose solution into graduated cylinder 4, 10 mL of starch solution into graduated cylinder 5 and 10 mL of distilled solution into graduated cylinder 6.
5.The test tube with the same number was added with 10 mL of each sample.
6.After that 5 drops of Benedict's solution was added to each test tube.
7.All the test tubes were heated in the hot water bath for 5 min.
8.The test tubes were then moved to the test tube rack using the test tube clamp. All the observations for each test tube were recorded, then the glassware was washed
1.Steps 3,4 and 5 were repeated from part A.
2. After that 5 drops of iodine solution was added to the test tube.